Analysis Note

ControlHuman placenta tissue lysate

Application

This Anti-Pleiotrophin Antibody, clone 3B10 is validated for use in IH, WB for the detection of Pleiotrophin.

Research Sub CategoryApoptosis - AdditionalDevelopmental Neuroscience

Research CategoryApoptosis & Cancer

Immunohistochemistry Analysis: A 1:500 dilution from a previous lot detected Pleiotrophin in papillary squamous cell carcinoma tissue.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Pleiotrophin (PTN) also called Neurite Growth-promoting Factor 1 (NEGF1) or HARP (Heparin affin regulatory peptide) is a new member of the heparin-binding neurotrophic factor family. PTN plays an important role in development processess and promotion of neurite extension. During embryonic development, pleiotrophin is expressed in the central and peripheral nervous system, lung, kidney, gut and bone. Pleiotrophin is also expressed by several tumor cells and is thought to be involved in tumor angiogenesis. PTN initiates an angiogenic switch in different cancer models and acts as an angiogenic factor. PTN does this through an interaction with endothelial cells to initiate proliferation, migration, and tube formation.

Immunogen

Recombinant protein corresponding to chicken Pleiotrophin.

Linkage

Replaces: MABD179

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing PBS without 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blot in human placenta tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Pleiotrophin in 10 µg of human placenta tissue lysate.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~20 kDa observed